Journal: Investigative Ophthalmology & Visual Science

Article Title: Ectoine Enhances Mucin Production Via Restoring IL-13/IFN-γ Balance in a Murine Dry Eye Model

doi: 10.1167/iovs.65.6.39

Figure Lengend Snippet: Antibodies Used in This Study

Article Snippet: MUC2 , CUSABIO , PA207869 , Rabbit , 1:3000.

Techniques:

Journal: Investigative Ophthalmology & Visual Science

Article Title: Ectoine Enhances Mucin Production Via Restoring IL-13/IFN-γ Balance in a Murine Dry Eye Model

doi: 10.1167/iovs.65.6.39

Figure Lengend Snippet: Mouse TaqMan Gene Expression Assays Used in This Study

Article Snippet: MUC2 , CUSABIO , PA207869 , Rabbit , 1:3000.

Techniques: Gene Expression

Journal: Investigative Ophthalmology & Visual Science

Article Title: Ectoine Enhances Mucin Production Via Restoring IL-13/IFN-γ Balance in a Murine Dry Eye Model

doi: 10.1167/iovs.65.6.39

Figure Lengend Snippet: Ectoine enhances production of secreted mucins in DS mice. ( A , C ) Representative images of IF staining showing the decreased production of MUC5AC ( A ) and MUC2 ( C ) in DS mice (DS+PBS) was recovered by ectoine eye drops (DS+Ect); Scale bars = 50 µm. The stitched images were made because the cornea and conjunctiva in some eyeball sections were too far apart to be captured on the same image. ( B , D ) IF intensity quantified the alternation of MUC5AC and MUC2 in 3 groups; each circle represents one eye. Data are shown as Mean ± SD, n = 6; the P values are shown in the graph as compared with the DS+PBS group.

Article Snippet: MUC2 , CUSABIO , PA207869 , Rabbit , 1:3000.

Techniques: Staining, Eye Drops

Journal: Investigative Ophthalmology & Visual Science

Article Title: Ectoine Enhances Mucin Production Via Restoring IL-13/IFN-γ Balance in a Murine Dry Eye Model

doi: 10.1167/iovs.65.6.39

Figure Lengend Snippet: Gene expression of MUC5AC, MUC2, MUC1, MUC4, MUC16 , and MUC15 by conjunctiva and cornea. ( A ) Gene expression of all 6 mucins in normal mice were detected at different mRNA levels based on the threshold cycle (Ct) value in real-time PCR with house-keeping gene GAPDH as an internal control. ( B ) The mRNA levels of all six mucins in conjunctiva of the three groups of mice. ( C ) The mRNA expression of MUC1, MUC4, and MUC16 by corneas in the three groups of mice. Data are shown as Mean ± SD, n = 6; the P values are shown in the graph as compared with the DS+PBS group.

Article Snippet: MUC2 , CUSABIO , PA207869 , Rabbit , 1:3000.

Techniques: Gene Expression, Real-time Polymerase Chain Reaction, Control, Expressing

Journal: Infection and Immunity

Article Title: Porcine Gastric Mucin Triggers Toxin Production of Enteropathogenic Bacillus cereus

doi: 10.1128/IAI.00765-18

Figure Lengend Snippet: Mucin degradation. (A) Mucin degradation by F837/76 cells (left) and culture supernatant (right). Lanes: M, PGM in Tris buffer; 1, PGM plus F837/76; 2, PGM plus F837/76 plus protease inhibitor; 3, PGM plus F837/76 plus protease inhibitor, EDTA free; 4, Tris buffer plus F837/76. Arrows highlight differing protein bands. (B) Growth of strain F837/76 in mMOD minimal medium after addition of 0.25% PGM. (C) Mucin degradation by F837/76 cells and supernatant (s). PGM type II and type III were used. (Left) Western blot with MUC2 specific antibody. (Right) Sypro Ruby staining of the same samples as a control. Arrows indicate MUC2 as well as differing protein bands.

Article Snippet: After 1 h of blocking in 3% casein-PBS, the membrane was incubated with 2 μg/ml NheB-specific MAb 1E11 ( 66 ) or with 1 μg/ml MUC2-specific antibody (abx177613; Abbexa Ltd.) for 1 h at room temperature.

Techniques: Protease Inhibitor, Western Blot, Staining